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ACL reconstruction with 6 strand hamstring allograft and Smith and Nephew Endobutton and RCI screw

Learn the ACL reconstruction with 6 strand hamstring allograft and Smith and Nephew Endobutton and RCI screw surgical technique with step by step instructions on OrthOracle. Our e-learning platform contains high resolution images and a certified CME of the ACL reconstruction with 6 strand hamstring allograft and Smith and Nephew Endobutton and RCI screw surgical procedure.
Have you ever wondered if you really need a tourniquet for ACL reconstruction surgery?
Alternatively, have you thought there may be a better way than ‘wasting’ the hamstring tails that are excised in a standard 4 strand semi-tendonosus and gracilis construct after tibial interference screw fixation?
If the answer to either of these questions is ‘yes’ then you should read on as in this technique I will describe my technique for reconstruction of a ruptured ACL with a 6 strand hamstring graft without a tourniquet. By using a 6 strand technique the cross-sectional diameter of the resultant graft is increased with a corresponding increase in absolute strength and thus load to failure limits as well. The absence of a tourniquet is a personal choice, but on systematic review and metanalysis (Kuo 2017) there are no downsides to not using a tourniquet and potentially benefits with reduced reduction in thigh girth and less bleeding post-operatively in the knee. Extrapolating from the arthroplasty world with no tourniquet there are also likely to benefits of reduced VTE risk by performing ACLR without tourniquet. There is no doubt that by increasing the strand numbers the graft diameter increases and this concept has been around since the mid 2000s (Krishna 2018 and Brown 2018).
For fixation in this case I am using the Smith & Nephew Endobutton (suspensory femoral fixation) and a Smith & Nephew metal RCI interference screw on the tibia. The RCI is a blunt threaded, 2mm cannulated metal screw which has a very long track record. The metal screws are easy to insert and remove and this is why I prefer a metal screw; the reason to remove a screw at a future date would either be for symptomatic tenderness on the anterior tibia or at revision if the graft ruptured. The endobutton is a 4mm x 12mm titanium button with 4 holes predrilled. The endobutton can be purchased separately eg for meniscal root repair, but it is usually supplied with a continuous loop of polyester around the central two holes. This allows a gentle loop through which the hamstring tendons are placed. In the two outer holes the endobutton comes pre-loaded with Ultrabraid (White) and Ethibond (Green) to provide a very strong pull and easier identification of the separate ends of the button to aid toggling on the lateral femoral wall.
ACL injuries are unfortunately a common injury in sporting trauma, often associated with injuries to the menisci and sometimes other ligaments. Chondral injuries less commonly occur at the time of injury, but left untreated ACL deficient knees will acquire meniscal or chondral injuries at a rate of 0.6 – 1% per month (Brambilla et al 2015). The chondral injuries are thought to occur as secondary damage when the knee has episodes of instability. Thus by 4 years (a 48% chance) approaching a one in two chance of meniscal or chondral injury which is known have significant long-term degenerative consequences on the knee.
The decision on whether to reconstruct an ACL rupture needs to be tailored to the patient, based on their future planned activity and their age (and thus their risk of subsequent secondary injury) or resultant instability after a trial of non-operative management. This is elegantly described in the Panther group output statement in 2020 by Diermeier; in young patients wanting to return to direction change activity then early ACLR reconstruction is advised, whereas those patients with no direction change may be treated non-operatively. If there is subjective instability after non-operative maximisation then ACLR is also indicated.
Readers will also find of interest the following associated OrthOracle operative techniques:
Anatomic ACL reconstruction using hamstring tendons ( Linvatec graft tensioner)
Anterior cruciate ligament reconstruction using a double-stranded hamstring graft
Patella tendon harvest for ACL reconstruction
Lemaire lateral extra-articular tenodesis for anterolateral rotational knee instability
Meniscal repair using Smith and Nephew fast-fix 360 system and knee arthroscopy
Arthroscopic lateral meniscal repair with fibrin clot and bone marrow aspirate -Smith and Nephew Fast Fix 360 system.

Brambilla L, Pulici L, Carimati G, Quaglia A, Prospero E, Bait C, Morenghi E, Portinaro N, Denti M, Volpi P. Prevalence of Associated Lesions in Anterior Cruciate Ligament Reconstruction: Correlation With Surgical Timing and With Patient Age, Sex, and Body Mass Index. Am J Sports Med. 2015 Dec;43(12):2966-73. doi: 10.1177/0363546515608483. Epub 2015 Oct 15. PMID: 26473010.
Brown CH Jr. Editorial Commentary: How to Increase Hamstring Tendon Graft Size for Anterior Cruciate Ligament Reconstruction. Arthroscopy. 2018 Sep;34(9):2641-2646. doi: 10.1016/j.arthro.2018.06.014. PMID: 30173804.
Diermeier T, Rothrauff BB, Engebretsen L, Lynch AD, Ayeni OR, Paterno MV, Xerogeanes JW, Fu FH, Karlsson J, Musahl V, Svantesson E, Hamrin Senorski E, Rauer T, Meredith SJ; Panther Symposium ACL Treatment Consensus Group. Treatment after anterior cruciate ligament injury: Panther Symposium ACL Treatment Consensus Group. Knee Surg Sports Traumatol Arthrosc. 2020 Aug;28(8):2390-2402. doi: 10.1007/s00167-020-06012-6. Epub 2020 May 9. Erratum in: Knee Surg Sports Traumatol Arthrosc. 2020 Sep 25;: PMID: 32388664; PMCID: PMC7524809.
Krishna L, Panjwani T, Mok YR, Lin Wong FK, Singh A, Toh SJ. Use of the 5-Strand Hamstring Autograft Technique in Increasing Graft Size in Anterior Cruciate Ligament Reconstruction. Arthroscopy. 2018 Sep;34(9):2633-2640. doi: 10.1016/j.arthro.2018.03.026. Epub 2018 May 24. PMID: 29804953.
Kuo LT, Yu PA, Chen CL, Hsu WH, Chi CC. Tourniquet use in arthroscopic anterior cruciate ligament reconstruction: a systematic review and meta-analysis of randomised controlled trials. BMC Musculoskelet Disord. 2017 Aug 22;18(1):358. doi: 10.1186/s12891-017-1722-y. PMID: 28830402; PMCID: PMC5567632.

INDICATIONS
Isolated ACL rupture with repairable meniscus tear
Young patients with ACL tears that wish to return to direction change sport or employment which needs knee stability – for example police, fire, military, building site workers (construction or engineers), farmers, roofers etc.
ACL rupture combined with multi-ligament injury being managed surgically.
Persistent instability in a non-operatively managed ACL deficient patient
SYMPTOMS & EXAMINATION
History
There is usually a clear cut history of a traumatic event in ACL injury. In this particular case there was a background of a football injury 3 years ago, without ever being able to return to football because of fear of giving way. There was then a traumatic fall down stairs with intermittent locking since the second event.
Usually the patient has a healthy knee prior to the event and they are involved in a sudden weight bearing direction change which is usually non-contact. During the event the patient frequently hears a loud ‘crack’ in the knee and is immediately aware of a significant injury. Sometimes patients describe this by holding up their two fists and showing movement between them – representing the femur and the tibia moving apart. The patient is then usually non-weight bearing and the knee becomes effused early, 75% will have an effusion within the first hour. The patient usually describes the pain as ‘excruciating’ and there is a usually certain visceral nausea associated with an ACL injury.
Sub-acutely patients with ACL injury complain of instability and then pain and swelling for a few days after the episode of ‘giving way’. Patients routinely avoid direction change activity as they know that their knee is not stable.
Sometimes well-muscled and well-conditioned patients can cope with isolated ACL injure, and after rehabilitation return to sport and then suffer another injury. During the second injury there is usually damage to the meniscus and sometimes the chondral surface, +/- other ligaments.
Locking (an inability to fully extend the knee) may be present particularly in recurrent injury.
Examination
Weight bearing status depends on the chronicity and associated injuries. Acutely most patients will struggle to fully weight bear.
Look for previous portals, the presence of swelling and bare in mind that when squatting the patient usually will be able to get lower on the non-injured side. There is normally a large knee effusion, but most commonly a moderate effusion detected on sweep testing by the time the patient is seen. The anterolateral side of the joint line around Gerdy’s tubercle is often tender. This represents strain of the anterolateral ligament or avulsion from the tibia.
Ligament examination:
A full ligament assessment must be made of the ACL, PCL, MCL, LCL and posterolateral corner.
Quads Active Test – thigh supported over the hand or arm of the examiner and ask the patient to extend their knee. Watch for movement of the tibia anteriorly as the Quads pull. The ACL deficient side moves more that the ACL intact side as the ACL resists the anterior translation of the proximal tibia and the force is then transmitted to extension of the leg distally.
Lachman: 30 degree knee flexion and anterior movement of the relaxed tibia. Look for side-side difference and the absence or presence of an endpoint.
Pivot Shift: This looks for the translation of the lateral tibia from reduced to subluxed (Pivot Jerk Test) or subluxed anteriorly to reduced (pivot shift). This relies on 3 forces – extension, valgus and internal rotation. However for the junior examiner this is ‘impossible’ as the patient will not relax and it should not be attempted. As experience increases this is the most valuable test. Remember to elevate the bed height so that the examiner does not flex the patient’s hip much (thus tightening hamstrings). Similarly ensure that the patient is recumbent – one pillow should suffice.
Anterior Drawer: The commonest taught test, performed with the knee flexed to 90 degrees and the hamstrings relaxed. Look for the relationship between the proximal tibia and the femoral condyles, like assessing a loss of step-off in a PCL deficient knee, this allows a degree of objectivity of the amount of movement during anterior translation.
The accepted specificity and sensitivities of these tests are:
Lachman – specificity 95% sensitivity 80-99%
Anterior drawer – specificity 75-95%; specificity 22-41% – risk of false positive
Pivot shift – specificity 98%; sensitivity 84-98.4% (35% in the awake patient) However this increases in experienced hands.
Record the findings using the following accepted grading conventions:
A or Grade 0 = Normal
B or Grade I = Slight Laxity, minimal translation, firm endpoint
C or Grade II = Moderate Laxity, moderate translation, firm or soft endpoint.
D or Grade III = Severe Laxity, no end-point, soft end-point
Remember that meniscal tears are common with ACL pathology. It is said that meniscal tears or chondral damage occurs at a rate of 1% of ACL-deficient patients per month
Meniscal tests such as the McMurray and Thessaly may be appropriate, though often in the acute setting the knee is too painful for these/
The McMurray test involves palpation of the joint line during passive flexion/extension of the knee joint with alternating internal and external rotation – a positive test equates to a palpable click on the joint line during the McMurray combined movement, usually with the medial joint on internal rotation and the lateral joint on external rotation.
The Thessaly test is a variation of the McMurray but with the patient weight-bearing through the injured knee; the patient should be supported ( I ask the patient to support their weight on the back of a chair) and then squat to 30 degrees and rotated externally (lateral meniscal provocation with a positive test if painful on the lateral joint line) or internally rotated ( medial meniscal provocation with a positive test if there is medial joint line pain).
IMAGING
In the acute setting with a history of trauma simple AP and lateral Radiographs should be performed to rule out fractures. In non-acute cases there may be a role for alignment views (for example if repair or root repair is being considered) or there is concern over significant varus with a combined Posterolateral Corner Injury
MRI however is by far the most important investigation to confirm the rupture of the ACL and to assess presence, site and morphology of any concomitant meniscal or chondral injury.
When learning to interpret MRI scans it is important to trace the ACL on both coronal, saggital and Axial scans – by having all three open together it is possible to build up a 3D picture of the ACL.
Perhaps the easiest scan slice to interpret is the Saggital T2 MRI – look for the presence of non-contiguous bone bruising showing where the posterolateral tibial plateau contacted the central lateral femoral condyle at the time of injury. There may be an associated chondral injury, subchondral fracture or just bone bruising in the knee at these sites. Look also for the ACL angle – typically the ACL should be at approximately 60 degrees to the tibia and in ACL deficiency the ACL will ‘fall down’ the PCL and sit at approximately 40 degrees.
ALTERNATIVE OPERATIVE TREATMENT
There is no alternative operative management if the decision to reconstruct the ACL has been made but there are different techniques of performing ACL reconstruction – most notably with the graft choice.
Graft Choice
Allograft: This should be reserved for multi-ligament reconstructions or cases of revision ACL where there are no suitable autografts available. Similarly if a patient is hypermobile and has ruptured a graft with good tunnel placement then an alternative donor tissue may be sensible. Allograft tissue may be all soft tissue eg Semitendonosus grafts, part bony eg TendoAchilles or quads tendon, or bone – tendon – bone such as a patella tendon allograft.
Autograft: The vast majority of cases should use the patient’s own tissue in order to reduce revision rates, reducing cost and reducing infections. The options are Hamstrings, Patella Tendon or Quadriceps tendon. All three are good grafts.There has been a lot in the literature comparing hamstrings against patella tendon (bone-tendon-bone or BTB) . From Magnussens’s systematic review in 2015 re-rupture rates at 10 years minimum range from 3.2% to 11.1% for the ipsilateral reconstructed side, but with a mean of 12.5% (ranging from 0.6% to 22.7%). In summary there is a slightly greater rate of re-rupture on the ipsilateral knee by using a hamstring graft, but if a BTB graft is used whilst the ipsilateral rupture rate is reduced, there is an increase in contra-lateral rupture rates.
Additional Lateral Extra-articular Tenodesis (LEAT)
Historically there were many different methods to stabilise the unstable knee with ACL deficiency by using extra-articular methods. More recently the addition of these techniques in addition to an anatomic intra-articular ACL reconstruction has taken off. This led to a multicentre RCT – the Stability study
The Stability Study has really confirmed the benefit of LEAT at 2 years compared to isolated ACLR. The re-rupture rate at 2 years was 11% for the ACLR group and 4% for the ACLR and LEAT group.
NON-OPERATIVE MANAGEMENT
In older patients this is the main treatment initially as the balance of risk to their menisci and chondral surfaces due to recurrent instability is less than the time-off work, time for rehab and surgical risks. There are very few young patients I would treat non-operatively.
In middle aged patients there needs to be a discussion and a shared decision – some will want early surgery and some will favour physiotherapy first and only adding in a reconstruction if there is residual instability which impacts in their lives.
Analgesia, crutches, braces and physiotherapy.
CONTRAINDICATIONS
Degenerative disease of the knee.
Active infection in the knee or at other sites in the body.
A patient who is unfit for anaesthetic
Ref:
Diermeier T, Rothrauff BB, Engebretsen L, Lynch AD, Ayeni OR, Paterno MV, Xerogeanes JW, Fu FH, Karlsson J, Musahl V, Svantesson E, Hamrin Senorski E, Rauer T, Meredith SJ; Panther Symposium ACL Treatment Consensus Group. Treatment after anterior cruciate ligament injury: Panther Symposium ACL Treatment Consensus Group. Knee Surg Sports Traumatol Arthrosc. 2020 Aug;28(8):2390-2402. doi: 10.1007/s00167-020-06012-6. Epub 2020 May 9. Erratum in: Knee Surg Sports Traumatol Arthrosc. 2020 Sep 25;: PMID: 32388664; PMCID: PMC7524809.

Pre-operative antibiotics
Side-support
Foot plate / sandbag
Arthroscopy stack
Shaved operative site
“Plan B” options:
consider preparation of contralateral leg for hamstring harvest
patellar tendon harvest equipment
supporting implants eg xtendobutton for inadvertent blow out of lateral femoral cortex; small fragment screws and washers or bone staples for secondary tibial fixation

Imaging – coronal MRI showing a bare lateral wall (1) indicating an ACL rupture from the lateral femoral condyle

Imaging – Axial MRI
Rupture ACL – (1)

Imaging – Sagittal MRI – notice the low lying ACL (1)

Preparation and selection of kit is important – I use a Linvatec hamstring stripper, Mitek reamers, Endobutton and Metal interference screw (Quick Start or RCI) for most ACL reconstructions
The Quick Start is from Innovate Orthopaedics. This has a novel double thread to optimise early and easy engagement.
The RCI is the original expanded head interference screw from Smith and Nephew. I still like the metal (Titanium) version as this allows easy visualisation on XR and easy removal should this be necessary at whatever timepoint in the future.

Operative set up is important – having the screen directly above the patient minimises the surgeon’s twisting which has ergonomic advantages
This image was taken during this ACL reconstruction case, but as can be seen on the screen, this particular element of the procedure is a meniscal repair.

The operative leg is supported with a side support and a foot holder, with the knee flexed to 90 degrees.I like to wrap the opposite leg which helps heat retention and prevents the leg dislodging off the table. It also stops adhesive drapes sticking to the Bair Hugger (which is carefully stuck to the patients’ skin to avoid air leak into the laminar flow operative zone)

Preparation with 2% chlorhexidine in alcohol and my standard arthroscopic draping including the foot in the preparation and then covering with a large sterile glove. The arthroscopic collection pouch can be part of your drape or alternatively these can be free-standing items which go over the drape.


Examination, formation of arthroscopic portals and diagnostic sweep is the first main event during an ACL reconstruction I like to harvest hamstrings through a nearly vertical incision – slightly oblique in the line of the saphenous nerve branches to try and minimise neuroma and numbness laterally.
For the portals I prefer nearly vertical for the high portals (anterolateral and anteromedial) and nearly horizontal for the low accessory medial portal.
Pure vertical or horizontal incisions are only extendable in one plane and by being oblique this gives the surgeon some flexibility or portal adjustment. In addition the cutaneous nerve branches run obliquely around the knee and parenthesis and neuroma formation are not unknown following arthroscopic surgery.
A pure horizontal low AM portal would minimise the risk to the medial meniscus but an oblique at 45 degrees is best for minimising cutaneous nerve damage to infra-patella branches of the saphenous nerve. Hence my compromise of near horizontal for the low accessory medial portal.


Pre-infiltration with adrenalinised local anaesthetic helps for reducing bleeding and improved analgesia helps the patient.
I use a multi-modal ‘cocktail’ of:
150ml of 0.1% Marcain with 0.6mg Adrenaline in 3x50ml syringes.
In one of the syringes we add:
30mg Ketorolac
10mg Morphine
The superficial infiltration performed prior to incision is Marcain and Adrenaline only.
I infiltrate around the knee – not into the knee cavity. Approximately 20ml to the medial side and 20 ml to the lateral side and 10ml around the Hamstring harvest site initially

The ACL injury is confirmed (1) and an accessory medial portal positioned by inserting a long needle (Venflon – labelled 4) along the track of the future drill for the femoral tunnel
Once the trajectory of the Venflon/needle is confirmed (from arthroscopic visualisation) not to conflict with the medial femoral condyle, but allowing the tip of the needle to touch the ACL footprint (site of future drilling) then the accessory medial portal can be made where the venflon/needle enters the skin.
I prefer a nearly horizontal incision for the accessory medial portal as described above.
I use an antegrade drilling technique from outside in, viewing from a high and very anterior anteromedial portal and drilling from a low and more medial accessory medial portal.
The importance of this step is to check the trajectory of the Venflon from the skin, past the medial condyle(2), allowing enough room for a reamer to pass without causing chondral damage, then up to the lateral wall close to the bifurcate ridge at the centre of the ACL origin (3).

Once the diagnostic sweep has been performed any meniscal or chondral injuries should be addressed. In this case a medial meniscal repair was needed.
This is covered in the following OrthOracle techniques.
Meniscal repair using Smith and Nephew fast-fix 360 system and knee arthroscopy
Arthroscopic lateral meniscal repair with fibrin clot and bone marrow aspirate using Smith and Nephew Fast Fix 360 system.

Some of the ACL stump is removed arthroscopically and the lateral femoral wall is partially cleared using a radiofrequency device . I use the Flo 50 from Smith Nephew as this has 3 settings for different applications
For the lateral wall clearance, I tend to remove very little tissue now, but during initial stages of learning this operation a clearer vision of the lateral wall will be required and training surgeons should be prepared to remove as much as necessary to achieve a good view.
I still ensure that the back of the notch is always seen.

I find the high power setting works very well and with this device if there is any bleeding, the coagulation function stops it efficiently. This is important as I do not use a tourniquet – all of these images are tourniquet free
This image is taken with the Flo 50 on high power setting, removing some of the ACL stump (1). Notice the bubbles (2)which need to be irrigated away to maintain vision using the hand pump or automatic irrigation pump.

After clearing of the lateral wall, image taken with the Flo 50 ‘turned off’.

The malleable intra-articular ruler (Smith Nephew) is fashioned by hand into a gentle curve to allow introduction from the antero-lateral portal and then aimed around the lateral femoral condyle to measure the depth of the lateral notch.
The obturator is aiming at the ‘half-way’ point (12) which is simply the depth of the notch on the lateral wall divided by two. This corresponds to the centre of the ACL.
This will be used in the next slides to position the Awl to define the start of the femoral tunnel.

With the scope (1) in the antero-medial portal, the awl (2) from the accessory medial mark the start of the femoral tunnel in the lateral condyleThe awl (‘chondral pick’ or ‘Steadman awl’) is used to do this.
The ruler is in my hand-3.

The depth of the lateral femoral wall is measured with the ruler
In this case 22mm from posterior cartilage to anterior cartilage.
The awl is inside the knee and ready to mark the start of the femoral tunnel at the ‘half way point’ (24mm/2 = 12mm)

The femoral starting hole for the drill has been formed by the awl (1)
This entry whole in the lateral femoral wall is at 12mm (1) This image has been taken directly from the screen intraoperatively and corresponds to the intra-articular photograph taken from the scope in the next slide…

Arthroscopic image close-up of the lateral wall with the femoral starter hole (1) and the malleable ruler still in place
Notice the Bifurcate Ridge (2) of the Lateral Femoral Condyle which divides the two main bundles of the ACL – the posterolateral (PL) and anteromedial (AM) bundles
The AM bundle is deep to the ridge and higher in the notch, whereas the PL bundle is shallower and lower.
I have positioned this starter whole just deep to the bifurcate ridge and biassed towards an AM position from the centre of the ACL femoral footprint.

The femoral tunnel is drilled to a diameter of 4.5mm across the lateral condyle from the pilot hole in the notch.The scope still in the anteromedial portal is then positioned to look down (light lead pointing up – 1) and the drill (2) is introduced into the knee from the accessory medial portal
This drill is a solid 4.5mm pointed-tip drill. The point fits into the pilot hole made by the awl previously.
The drill needs to be held firm and the scope maintaining vision of the drill in the pilot hole as the knee is then flexed by the assistant into the highest flexion possible in the next slide.
For a left ACLR, I would normally hold the drill in my left hand and the scope in my right hand (with ring finger touching the scope-portal aperture for best control) but we have moved our hands away for this photograph.

The knee is hyperflexed to allow accessory medial portal drilling of the femoral tunnel from the notch through the far lateral cortex.
The advice to the assistant is to slowly draw the foot as close to the patient’s thigh as possible – for this left ACLR, by using the assistant’s left hand. It is often advisable to apply pressure on the patient’s thigh (with the assistant’s opposite hand -right hand here) to allow the hyper flexed position we need for drilling of the femoral tunnel.
There are alternative strategies such as flexible reamers or retrograde drilling if you find this step hard.
It is important to visualise the medial femoral condyle with the arthroscope before drilling to prevent chondral damage form the drill.

Intra-articular view of the 4.5mm drill (1) in the pilot hole and about to drill the femoral tunnel in the lateral femoral condyle (2)

The length of the femoral tunnel is measured with the Endobutton Depth Gauge, 1.This obviously depends on the size of the patient, but a range of 30-45mm is a useful guide.

The femoral tunnel is measured at 42mm – where the ruler disppears into the lateral femoral condyle (42) on the image.
Each laser-marked division is 2mm.

Harvest the hamstring graft, either just Semi-Tendonosis (ST) or both ST and Gracilis. The incision is centred over the insertion of the medial hamstrings, anterior to the superficial MCL.Whilst there is a size difference between patients a rough guide is useful to learn in terms of your own fingers! I use a distance of 3 fingers down from the tibia-femoral joint line and 2 fingers medial to the lateral border of the tibial tubercle. This forms the starting point for palpation of the hamstring insertion which feels like a hollow then a bump moving from proximal to distal.
I prefer a slightly oblique incision as explained earlier to reduce the chance of nerve damage and for good cosmesis.
The decision on harvesting ST alone or Gracilis too, depends on the size and length of the ST harvested and the size of the patient. As a guide, aim for a total graft construct of 100mm including the suspensory fixation (15-20mm Endobutton usually for me). Thus if ST can be folded over 4 times and achieve 80mm, there is sufficient length.
If this is not possible then 3 strands of ST with the addition of Gracilis (2 0r 3 strands) would make a 5 or 6 strand graft which will be in the region of 80-90mm long and 8-9.5mm in diameter. With the addition of a button/loop the total length is then 100-110mm.

A swab is used to cover the arthroscopic portals and another swab introduced via a Lehey clamp is used to sweep away the fat from the underlying sartorius fascia

The fascia is then divided transversely immediately proximal to the palpable ‘pes asnerinus’ (or goose’s foot) which represents the insertion of the sartorius, gracilis and semi-tendonosus tendons.

By using a curved haemostat (the Lehey is perfect for this task) the medial hamstring insertion is delivered from the undersurface of the Sartorius fascia and into the incision.

Placing a swab between the tendon and the surgeon’s finger is a useful step to improve the comfort of the next step for the surgeon!

I like to place my finger under the tendon during harvest with a stripper – this allows me to check on the tension being applied to the insertion of the hamstrings by the stripper.
If the force is too large, then the hamstring insertion can be avulsed and the tendon will retract proximally.

Using curved dissecting scissors the vinculae (which supply the tendons) are divided close to the tendon with the curve of the scissors away from the tendon
There is a risk of damaging the tendon during this step, but without this removal of vinculae there is an even greater chance of tendon damage and transection during the stripping stage below.

By pulling the tendon and palpating the skin of the distal thigh medially (1) it is possible to detect any retained vinculae before stripping.

Carefully insert the tendons (within the incision) into the mouth(2) of the Linvatec hamstring stripper (1)with the guard closed.The guard of the Linvatec stripper is closed by rotating the lever on the handle to ‘closed’ which is the middle position – see next slide marked ‘1’.

Steady pressure is applied to the Hamstring stripper with one hand (right) whilst the other hand (left) is holding the tendon at its insertion and protected by a swab.
The stripper usually inserts up to the handle and then the tendon separates from the muscle body without the need for a formal ‘cut’ which is achieved by rotating the trigger on the stripper.
The stripper usually inserts to the handle and then the tendon separates from the muscle body without the need for a formal ‘cut’ which is achieved by rotating the trigger on the stripper from ‘closed’ (1) to ‘cut’ (2).

Using the hamstring stripper as a target for local anaesthetic infiltration (LAI) ensures that the local anaesthetic is at the site of hamstring tendon removal

The Semi-tendinosus tendon (1) is then first withdrawn from its proximal end as the stripper is removed out of the woundFurther anaesthetic is placed around the anterior soft tissues.

The Hamstring stripper step is then repeated to harvest the gracilis tendonNotice how I am holding the ST tendon in my left hand as well as tensioning the gracilis as the stripper is introduced.

By using one blade of a pair of a pair of scissors, all attached muscle is stripped off the tendons once harvested.Similarly vinculae are removed to leave smooth, long tendons.
Notice the position of the tendon graft (1) held between my thumb (2) and one blade of a pair of scissors (3)

A six strand graft is then made by suturing the end of the two tendons to an Endobutton (1) and then looping the two tendons around a nylon tape (2) and then passing back through the endobutton, before clamping the two free ends back to the nylon tape with a haemostat (3)The decision on harvesting ST alone or Gracilis too, depends on the size and length of the ST harvested and the size of the patient. As a guide aim for a total graft construct of 100mm including the suspensory fixation (15-20mm Endobutton usually for me).
Three strands of ST with the addition of 3 strands of Gracilis (2 0r 3 strands) has made a 6 strand graft which will be in the region of 80-90mm long and 8-9.5mm in diameter. With the addition of a button/loop (1) the total length is then 100-110mm. The Nylon tape (2) provides the necessary traction for the graft outside the tunnel to allow fixation under tension without unnecessary removal of any tendon.
The Haemostat (curved artery clip – 3) is securing the free tendon ends and the Nylon tape together, but it is not catching the looped tendon

I use an ultra-strong braided suture to construct the graft. Notice that after initially suturing the graft to the button I leave the end long (1) and the FiberWire suture still attached (2)
I am using number 2 FiberWire (Arthrex) here. Alternative sutures would be Number 2 Ultrabraid or Orthocord. In other words a high tensile non-absorbable suture.

The graft is secured onto the button first, looping the suture around the button and for 2-3 throws through the graft, then back through the button and finally tied off and cut.

Then the trailing (adjacent the nylon loop) end of the graft is sutured, but avoiding going through the nylon tape – this allows the tape to be pulled out at the end after fixation.

Once sutured with endobuttons sizing of the Graft is performed by matching the graft diameter to known sized tubes.Here there is an 8.5mm and 9mm tube placed over an arthroscopic grabber – and the leading sutures of the endobutton are placed into the clasps of the grabber to allow passage of the graft through the tubes.
This stage may need to be repeated, but with practice the surgical hand becomes an excellent guide of girth!

This is the Mitek sizing device which is useful for larger grafts
It also has slits in the side of the cylinders to allow the graft pull-through sutures to be slotted in front the side, removing the need for exchange with an arthroscopic grabber as above.
The down-side of the slotted graft-measurer is that the graft can expand into the slot…so the graft may be a little larger than the diameter of the tube, thus compromising the accuracy of the sizing
Consequently be careful if the graft feels tight in the chosen size slot……the graft is actually bigger!

It is worth keeping a sizing tube over the tightest part of the graft – usually the button/graft junction or the tail end. I often use 2 tubes to cover the whole graft – and then place it in a graft bath of saline and 1g of Vancomycin. These are sizing tubes from an RCI reaming set, but many others are available such as the Arthrex Graft compression tubes.
Notice the swab in the ‘Vanc-bath’ which will be wrapped around the graft in the next image.

The graft is then wrapped up in the vancomycin-soaked swab and the sutures gently wound around the swab…..

For safe keeping, I place the wrapped Graft into a surgical glove

The glove is tied closed and placed in a receiver – this removes a risk of graft contamination, particularly the potential disaster of a graft being dropped on the floor during the next steps of the operation.

The femoral tunnel is then reamed over a Beath pin to the corresponding size to the femoral diameter of the graft, to create a femoral socket.The minimum length of this socket depends on the relative length of the femoral tunnel and the loop length of the suspensory fixation.
However the simplest solution is to ream up to the cortex, but not through the lateral femoral cortex.
If that happens then either a lateral wall alternative fixation (post screw and tie down of suture ends from the button or a Swivel-lok from Arthrex) or a femoral interference screw (to create aperture fixation) should be used.

The knee must be at the same position of flexion (hyerflexion) as when the femoral tunnel was drilled, so that the reamer (1) does not eccentrically remove bone.
Notice how the assistant’s arms are away from the knee and thus outside the scope of this image.
The assistant is holding the ankle with left hand and patient’s thigh with their right hand. The assistant’s right hand should push down on the thigh (2) to hold the knee locked in this position during reaming.


The bone swarf from reaming is then removed .
I use a sucker tip (2) to collect the bone swarf. Simply introduce the sucker tip into the accessory medial portal and obstruct the draining end (and close the suction control hole where applicable). Raise the intra-articular pressure by a couple of hand pumps or allow the pressure to raise with the arthroscopic pump, then release the sucker to drain freely.
It is worth tapping the popliteal fossa (1) as there is often a collection of bone swarf which falls posteriorly.

A nylon pull through loop is passed from the accessory medial portal through the femoral tunnel.This can be looped on itself and secured outside the knee or alternatively the loop simply withdrawn from the lateral side so that the loop (1) is just inside the femoral tunnel (2) and away from potential snagging by the tibial reamer.

The ‘Gold Guide’ – or Smith and Nephew tibial elbow aimer (1) is then prepared to identify and drill the tibial tunnel.
As the surgeon you will be asked what angle you want this setting to? A sensible answer is between the 50 and 55mm markings (52.5 degrees!).
In reality I virtually always change this angle during introduction of the device (using the silver tightening wheel 3) as this allows independent aperture identification intra-articularly and then the guide sleeve (2) is positioned within the hamstring harvest wound on the bare area of the tibia.

The Smith and Nephew tibial elbow aimer is set between 50-55 usually, inserted into the anteromedial portal in preparation for tibial tunnel drilling. Introduction of the Gold Guide (1) is best done upside down, using the anteromedial portal, then rotate this round into the correct position.
Once the tip or elbow aimer are introduced into the knee the guide (1) is rotated 180 degrees so that the tibial aiming guide (2) is sitting within the hamstring harvest incision (3)

The elbow aimer is positioned relative to the ACL remnant, the medial tibial spine (palpated arthroscopically), the PCL and the anterior horn of the lateral meniscus (both visualised)

Tibial tunnel preparation – deciding where to drill1 = PCL
2 = Lateral Wall
3 = Gold Guide Elbow Aimer.

The Beath (passing) pin is inserted through the tibial guide, aiming for the centre of the ACL stump and the lateral meniscal anterior root.The guide is then removed leaving the pin in place in the tibia.

The passing pin (1) is inserted in the centre of the ACL stump (2).
Another useful landmark (although not in this image) is the lateral meniscal anterior root.

The tibial passing pin is reamed with a small diameter reamer – eg 5mm

Reversing the Beath pin prior to removal of the first reamer will allow fine tuning of the final tibial tunnel placementTo achieve this fine-tuning, the flat eyelet of the passing pin is held intra-articualrly by a clamp (I use a Lehey from a medial portal). The most proximal end of the tibial tunnel can then be biassed by moving the pin within the 5mm tunnel. For example the tunnel could be pushed more medial and posterior by manipulating the Lehey clamp which in turn is holding the Beath pin. The correct size reamer to match the graft is then taken and the tunnel will be moved towards the posterior and medial sides of the original tunnel.

The Beath pin is held with a Lehey clamp inside the knee and the ideal tibial tunnel position chosen within the 5mm diameter already reamed.

The tibial tunnel is expanded with reamer size corresponding to the graft diameter

Clearance of soft tissue around the tibial tunnel entrance – using a large arthroscopic punch is ideal.

This small amount of soft tissue (removed by the arthroscopic punch -1) comes from around the entrance of the tibial tunnel anteriorly.
Notice my finger (2) for appreciation of the scale.
If this is not removed it can block graft passage later on.

The nylon loop is retrieved from the femoral tunnel and pulled down the tibial tunnelThe loop is retrieved from the femoral tunnel (by an arthroscopic hook or a meniscal grabber) into the knee and then retrieved from the anterior side of the tibial tunnel by the same instrument placed up the tibial tunnel into the knee.

The 6 strand graft is removed from the Vanc bath and glove and covered with Instillagel
Instillagel is a proprietary brand of sterile lubricating jelly with Lidocaine, commonly used for urinary catheter insertion.

More Instillagel is applied into the tibial tunnel.

The hamstring graft is introduced through the Nylon loop and then shuttled across both tibial and femoral tunnels….

The graft is pulled across using the white ultrabraid (1) which is preloaded on the Endobutton.

If more traction is needed to pull the graft through, then a Kocher clamp (1) may be clipped onto the Ultrabraid (away from the skin) and then the ultrabraid wound around the clamp to achieve a short lever which can then be traction from the lateral side.
Please note this image is used to show cutting one green and one white suture (2) before pulling the other two out, but the Kocher clamp used to pull the graft up the the tunnels is still attached and hence using the image now.

Once the Graft is fully inserted into the femoral tunnel, the green (Ethibond) sutures can then be pulled to toggle the endobutton from parallel to the femoral tunnel to perpendicular, and then the graft tensioned, pulling from the tibial side.It is sensible to cycle the knee from 0-70 degrees (approximately 10-20 times) to ensure that the endobutton is sat down on the lateral femoral cortex and the graft is appropriately tensioned before moving onto fixation of the tibial side….

Two of the pull through sutures (one white, one green) are cut flush to the skin and then the other two sutures removed
The graft can then be pulled back down the tibia and the knee cycled to ensure that the graft is tensioned and the endobutton is sitting on the lateral femoral cortex.

A tibial guide wire for the chosen interference screw is inserted in the tibial tunnel on the lateral side of the graft.
This forces the graft medially to give more obliquity and therefore better rotational control.

Tibial fixation is then completed by insertion of the RCI screw along the guide wire.A metal RCI screw (1) is inserted over the guide wire with traction on the graft using the Nylon tape (2).
I place the knee in approximately 70 degrees – with the foot on the foot roll to start the interference screw, but then straighten the knee and apply a reverse Lachman as in the next image

Reverse Lachman test is used to tension the graft as the tibial interference screw is tightened home.The assistants’ hands (1&2) perform a reverse Lachman. Hand 1pushes anteriorly and hand 2 pushes posteriorly.
The surgeons’ hands (3&4) tension the graft and insert the interference screw. For this left knee I am pulling the graft (Nylon tape) with my left hand (3) and inserting a metal RCI screw with my right hand (4).

Examination of the knee to ensure that stability is restored after fixation of the graft.I use a Pivot Shift, Anterior Drawer and Lachman test at this stage.
The key for me is abolition of the pivot and full range of the knee.

Deep dermal closure with absorbable suture – I use 2-0 vicryl

Good skin closure is essential to prevent wound complications.
Subcuticular (absorbable monofilament such as 3-0 Monocryl) and steristrips are applied

Occlusive dressings are applied with the knee in flexion to help with early mobility and allow early showering.

Crepe (criss-crossed) on top of a thinly applied wool bandage from ankle to thigh.

Post-operative XR – AP
The endobutton is sitting down on the lateral femoral cortex
The tibial interference screw is not prominent in the joint.

Post-operative XR – Lateral
The tibial RCI screw is sitting within the tibia, not prominent anteriorly or intra-articularly.
The graft is sitting posterior and medial to the screw.

We use two protocols in Bristol:
Early Accelerated
Slow stream ACL Rehabilitation.
The decision as to which pathway I follow depends on the patient (eg under 20 year olds or hypermobility) as well as the operative procedures (additional meniscal repairs requiring a period of reduced weight bearing such as Root Tears or Radial Meniscal Tears).

Early Accelerated Pathway
Daycase
Full Weight Bear
Crepe bandage reduced prior to discharge and single tubigrip applied
Crutches for balance – usually only for 1-2 weeks
Physiotherapy on the day, but mostly for safety reasons to allow mobility. Planning of future physiotherapy from 1-2 weeks.
Patient removes their own dressings at 2 weeks – any concerns they should phone the surgical team back and request review – this is very rare.
Outpatient Review – 6-8 weeks with Xray to document implants and tunnels

Slow Stream Rehabilitation
Brace 0-90 4 weeks – this may be modified to a lower flexion angle in meniscal repair cases
Crutches Touch Weight Bearing for 2 weeks
Physiotherapy starts at the same 1-2 week time interval, but is on a slower rehabilitation schedule to reduce graft loading.
Patient removes their own dressings at 2 weeks – any concerns they should phone the surgical team back and request review – this is very rare.
Outpatient Review – 6-8 weeks with Xray to document implants and tunnels

The aim of ACL reconstruction is to restore knee function to full activity. In reality though this is a multifactorial goal. For example patients may decide not to return to their pre-injury level for work/family/age reasons. However the aim should be full function.
Time scales for return to functions vary depending on philosophy. This is a guide:
First 1-2 weeks – normal weight bearing
2-4 weeks for driving (unless left leg and automatic car then this is much earlier)
4-6 weeks – quiet knee with minimal effusion and near full range.
6 weeks – the weakest time point of the graft! From this time there will start to be a slow increase in strength up to 2 years.
3 months – light sport activity under physio guidance. Cylcic loading can now start.
6 months – sports-specific training starts. Some authors allow return to sport. Injury prevention work for both knees
9 -12 months – Increasing sports activity. I prefer to aim for around 12 months for return to competitive sport. The graft continues tom improve in the 2nd year and re-rupture rates will diminish after 24 months, but the 12-24 month improvement is small, compared to the 9-12 month reduction in re-rupture rates and therfore I suggest around 12 months as long as the patient has ‘Knee Confidence’
Regarding re-rupture rates these vary – I have quoted two long-term papers for hamstring and BTB use below, although from Magnussens’s systematic review in 2015 re-rupture rates at 10 years minimum range from 3.2% to 11.1% for the ipsilateral reconstructed side, but with a mean of 12.5% (ranging from 0.6% to 22.7%). In summary there is a slightly greater rate of re-rupture on the ipsilateral knee by using a hamstring graft, but if a BTB graft is used whilst the ipsilateral rupture rate is reduced, there is an increase in contra-lateral rupture rates.
For all UK ligament surgeons, the National Ligament Registry (www.uknlr.co.uk) is an excellent way to assess personal results and then comparing yourself to the National Cohort. I strongly encourage use of this – Please take my lead and encourage others to use of this.
References:
The National Ligament Registry (www.uknlr.co.uk)
Amis AA, Jakob RP. Anterior cruciate ligament graft positioning, tensioning and twisting. Knee Surg Sports Traumatol Arthrosc. 1998;6 Suppl 1:S2-12. doi: 10.1007/s001670050215. PMID: 9608456.
Brambilla L, Pulici L, Carimati G, Quaglia A, Prospero E, Bait C, Morenghi E, Portinaro N, Denti M, Volpi P. Prevalence of Associated Lesions in Anterior Cruciate Ligament Reconstruction: Correlation With Surgical Timing and With Patient Age, Sex, and Body Mass Index. Am J Sports Med. 2015 Dec;43(12):2966-73. doi: 10.1177/0363546515608483. Epub 2015 Oct 15. PMID: 26473010.
Brown CH Jr. Editorial Commentary: How to Increase Hamstring Tendon Graft Size for Anterior Cruciate Ligament Reconstruction. Arthroscopy. 2018 Sep;34(9):2641-2646. doi: 10.1016/j.arthro.2018.06.014. PMID: 30173804.
Diermeier T, Rothrauff BB, Engebretsen L, Lynch AD, Ayeni OR, Paterno MV, Xerogeanes JW, Fu FH, Karlsson J, Musahl V, Svantesson E, Hamrin Senorski E, Rauer T, Meredith SJ; Panther Symposium ACL Treatment Consensus Group. Treatment after anterior cruciate ligament injury: Panther Symposium ACL Treatment Consensus Group. Knee Surg Sports Traumatol Arthrosc. 2020 Aug;28(8):2390-2402. doi: 10.1007/s00167-020-06012-6. Epub 2020 May 9. Erratum in: Knee Surg Sports Traumatol Arthrosc. 2020 Sep 25;: PMID: 32388664; PMCID: PMC7524809.
Getgood AMJ, Bryant DM, Litchfield R, Heard M, McCormack RG, Rezansoff A, Peterson D, Bardana D, MacDonald PB, Verdonk PCM, Spalding T; STABILITY Study Group, Willits K, Birmingham T, Hewison C, Wanlin S, Firth A, Pinto R, Martindale A, O’Neill L, Jennings M, Daniluk M, Boyer D, Zomar M, Moon K, Pritchett R, Payne K, Fan B, Mohan B, Buchko GM, Hiemstra LA, Kerslake S, Tynedal J, Stranges G, Mcrae S, Gullett L, Brown H, Legary A, Longo A, Christian M, Ferguson C, Mohtadi N, Barber R, Chan D, Campbell C, Garven A, Pulsifer K, Mayer M, Simunovic N, Duong A, Robinson D, Levy D, Skelly M, Shanmugaraj A, Howells F, Tough M, Spalding T, Thompson P, Metcalfe A, Asplin L, Dube A, Clarkson L, Brown J, Bolsover A, Bradshaw C, Belgrove L, Millan F, Turner S, Verdugo S, Lowe J, Dunne D, McGowan K, Suddens CM, Declercq G, Vuylsteke K, Van Haver M. Lateral Extra-articular Tenodesis Reduces Failure of Hamstring Tendon Autograft Anterior Cruciate Ligament Reconstruction: 2-Year Outcomes From the STABILITY Study Randomized Clinical Trial. Am J Sports Med. 2020 Feb;48(2):285-297. doi: 10.1177/0363546519896333. Epub 2020 Jan 15. PMID: 31940222.
Krishna L, Panjwani T, Mok YR, Lin Wong FK, Singh A, Toh SJ. Use of the 5-Strand Hamstring Autograft Technique in Increasing Graft Size in Anterior Cruciate Ligament Reconstruction. Arthroscopy. 2018 Sep;34(9):2633-2640. doi: 10.1016/j.arthro.2018.03.026. Epub 2018 May 24. PMID: 29804953.
Kuo LT, Yu PA, Chen CL, Hsu WH, Chi CC. Tourniquet use in arthroscopic anterior cruciate ligament reconstruction: a systematic review and meta-analysis of randomised controlled trials. BMC Musculoskelet Disord. 2017 Aug 22;18(1):358. doi: 10.1186/s12891-017-1722-y. PMID: 28830402; PMCID: PMC5567632.
Magnussen RA, Meschbach NT, Kaeding CC, Wright RW, Spindler KP. ACL Graft and Contralateral ACL Tear Risk within Ten Years Following Reconstruction: A Systematic Review. JBJS Rev. 2015 Jan 20;3(1):01874474-201501000-00002. doi: 10.2106/JBJS.RVW.N.00052. PMID: 27501023.
Murray JR, Lindh AM, Hogan NA, Trezies AJ, Hutchinson JW, Parish E, Read JW, Cross MV. Does anterior cruciate ligament reconstruction lead to degenerative disease?: Thirteen-year results after bone-patellar tendon-bone autograft. Am J Sports Med. 2012 Feb;40(2):404-13. doi: 10.1177/0363546511428580. Epub 2011 Nov 23. PMID: 22116668.
Robinson J, Inderhaug E, Harlem T, Spalding T, Brown CH Jr. Anterior Cruciate Ligament Femoral Tunnel Placement: An Analysis of the Intended Versus Achieved Position for 221 International High-Volume ACL Surgeons. Am J Sports Med. 2020 Apr;48(5):1088-1099. doi: 10.1177/0363546520906158. Epub 2020 Mar 17. PMID: 32182091.
Salmon LJ, Russell VJ, Refshauge K, Kader D, Connolly C, Linklater J, Pinczewski LA. Long-term outcome of endoscopic anterior cruciate ligament reconstruction with patellar tendon autograft: minimum 13-year review. Am J Sports Med. 2006 May;34(5):721-32. doi: 10.1177/0363546505282626. Epub 2006 Jan 6. PMID: 16399931.


Reference

  • orthoracle.com
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